![Overlay histogram showing THP1 cells stained with ab118044 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118044, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/26190_ab118044-6-ab118044FC.jpg)
Overlay histogram showing THP1 cells stained with ab118044 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118044, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![All lanes : Anti-S100A12 antibody [1D1] (ab118044) at 1/500 dilutionLane 1 : HEK293T cells transfected with the pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with the pCMV6-ENTRY S100A12 cDNALysates/proteins at 5 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/26191_S100A12-Primary-antibodies-ab118044-1.jpg)
All lanes : Anti-S100A12 antibody [1D1] (ab118044) at 1/500 dilutionLane 1 : HEK293T cells transfected with the pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with the pCMV6-ENTRY S100A12 cDNALysates/proteins at 5 µg per lane.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using 1/150 ab118044.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using 1/150 ab118044.
Immunohistochemical staining of paraffin-embedded Human liver tissue using 1/150 ab118044.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using 1/150 ab118044.