S100A12

Gene Symbol S100A12
Entrez Gene 6283
Alt Symbol CAAF1, CAGC, CGRP, ENRAGE, MRP-6, MRP6, p6
Species Human
Gene Type protein-coding
Description S100 calcium binding protein A12
Other Description EN-RAGE|S100 calcium-binding protein A12 (calgranulin C)|calcitermin|calcium-binding protein in amniotic fluid 1|calgranulin C|calgranulin-C|extracellular newly identified RAGE-binding protein|migration inhibitory factor-related protein 6|neutrophil S100 protein|protein S100-A12
Swissprots P83219 P80511 Q7M4R1 Q5SY66
Accessions BAA12030 CAB94792 EAW53332 P80511 BC070294 AAH70294 D49549 BAA08497 D83664 BAA12036 HQ257882 ADR82637 X97859 CAA66453 NM_005621 NP_005612
Function S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+)
Subcellular Location Secreted {ECO:0000269|PubMed:22811950}. Cytoplasm {ECO:0000269|PubMed:22811950}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:22811950}. Cell membrane {ECO:0000269|PubMed:22811950}; Peripheral membrane protein {ECO:0000269|PubMed:22811950}. Note=Predominantly localized in the cytoplasm. Upon elevation of the intracellular calcium level, translocated from the cytoplasm to the cytoskeleton and the cell membrane. Upon neutrophil activation is secreted via a microtubule-mediated, alternative pathway.
Tissue Specificity Predominantly expressed by neutrophils, monocytes and activated macrophages. Expressed by eosinophils and macrophages in asthmatic airways in regions where mast cells accumulate. Found in high concentrations in the serum of patients suffering from various inflammatory disorders, such as rheumatoid arthritis, psoriatic arthritis, Crohn's disease, ulcerative colitis, and Kawasaki disease.

Calgranulin C (C-20) - sc-8116 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Calgranulin C (C-20): sc-8116. Western blot analysis of Calgranulin C expression in DMSO-treated HL-60 whole cell lysate.

Calgranulin C (19F5) - sc-101347 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µl ascites
Host Mouse
Clonality Monoclonal

Calgranulin C (H-40) - sc-50451 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-S100A12 antibody - ab37657 from Abcam

Prices $384.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal
Anti-S100A12 antibody (ab37657) + 200 ng recombinant human S100A12Secondaryanti-rabbit IgG alkalind phosphatase conjugate

Anti-S100A12 antibody - ab196740 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling S100A12 with ab196740 at 1/200 dilution.

Anti-S100A12 antibody - ab172268 from Abcam

Prices $376.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-S100A12 antibody (ab172268) at 1/500 dilutionLane 1 : S100A12-transfected 293T cell line lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-S100A12 antibody - ab192422 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-S100A12 antibody [19F5] - ab50250 from Abcam

Prices $370.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone 19F5

Anti-S100A12 antibody [1D1] - ab118044 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1D1
Overlay histogram showing THP1 cells stained with ab118044 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118044, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-S100A12 Antibody - LS-C93436 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-S100A12 Antibody (clone 1F10) - LS-C139078 from LifeSpan Bioscience

Prices $355.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 1F10

Anti-S100A12 Antibody (aa1-92, PE) - LS-C182943 from LifeSpan Bioscience

Prices $635.00
Sizes 100 tst
Host Goat
Clonality Polyclonal

Anti-S100A12 Antibody (clone 19F5) - LS-C153339 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 19F5

Anti-S100A12 Antibody (aa1-92, Biotin) - LS-C299098 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-S100A12 Antibody (aa1-92, FITC) - LS-C302766 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-S100A12 Antibody - LS-C341576 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-S100A12 / CGRP Antibody - LS-C343755 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-S100A12 Antibody - LS-C141901 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
S100A12 Antibody - Detection of S100A12 in HeLa whole cell extracts.

Anti-S100A12 Antibody (aa1-92) - LS-C296306 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant S100A12 / CGRP.

Anti-S100A12 / CGRP Antibody - LS-C343756 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Sheep
Clonality Polyclonal
Immunohistochemistry: Vibratome (70 mu m) sections of rat spinal cord immunostained with sheep polyclonal antiserum to CGRP at a dilution of 1:1000. Negative images of fluorescence detection procedure (Alexa488 conjugated secondary antibody). Micrograph courtesy of Professor Andrew Todd (University of Glasgow).

Anti-S100A12 Antibody - LS-C335557 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human thyroid cancer using S100A12 antibody at dilution of 1:200 (400x lens).

Anti-S100A12 / CGRP Antibody (clone 1D1) - LS-C337273 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 1D1
IHC of paraffin-embedded Human lymph node tissue using anti-S100A12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).

EN-RAGE/S100A12 Antibody [Unconjugated] - AF1052 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Western blot shows lysate of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EN‑RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1052) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EN‑RAGE/S100A12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

EN-RAGE/S100A12 Antibody - NB110-90136 from Novus Biologicals

Prices $99.00, $279.00
Sizes 25 µl, 100 µl
Host Rabbit
Clonality Polyclonal

EN-RAGE/S100A12 Antibody (1D1) - NBP2-03681 from Novus Biologicals

Prices $379.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1D1
Western Blot: S100A12 Antibody (1D1) [NBP2-03681] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100A12 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A12.
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