All lanes : Anti-SAE1 antibody [EPR15397(B)] (ab185949) at 1/1000 dilutionLane 1 : Hela cell lysateLane 2 : Jurkat cell lysateLane 3 : MCF7 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-SAE1 antibody [EPR15397(B)] (ab185949) at 1/10000 dilution + 293 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-SAE1 antibody [EPR15397(B)] (ab185949) at 1/1000 dilutionLane 1 : Raw264.7 cell lysateLane 2 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunofluorescent analysis of -20℃ acetone-fixed HeLa cells labeling SAE1 with ab185949 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counter stain: Dapi (blue).
Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 cells labeling SAE1 with ab185949 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counter stain: Dapi (blue).