Anti-SAE1 antibody [EPR15398]

• Supplier: Abcam
• Catalog: ab185552
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR15398
• Applications: WB ICC/IF ICC/IF FC IP
• Species Reactivities: Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SAE1 aa 200-300
• Description: Rabbit Monoclonal
• Gene: SAE1
• Conjugate: Unconjugated

Product images

Immunoprecipitation from 293 cells using ab185552 at 1/50 dilution.  Secondary ab: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.  Blocking buffer: 5% NFDM/TBST.

Flow cytometry analysis of HeLa cells using ab185552 at 1/230 dilution.  Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution.  Fixative: 2% paraformaldehyde.  Isotype control: Rabbit monoclonal IgG.

Immunofluorescence analysis of MCF-7 cells labeling SAE1 with ab185552 at 1/500 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi.

Immunofluorescence analysis of HeLa cells labeling SAE1 with ab185552 at 1/500 dilution.  Secondary ab: Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution.  Fixative: 4% paraformaldehyde.  Counter stain: Dapi.

All lanes : Anti-SAE1 antibody [EPR15398] (ab185552) at 1/10000 dilutionLane 1 : Hela cell lysateLane 2 : Jurkat cell lysateLane 3 : 293 cell lysateLane 4 : MCF-7 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution