All lanes : Anti-SAMHD1 antibody (ab192595) at 1/1000 dilutionLane 1 : HepG2 l(Human liver hepatocellular carcinoma) cell lysateLane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysateLane 3 : THP-1 (Human monocytic leukemia cells) cell lysateLane 4 : MCF7 (Human breast adenocarcinoma cell line) cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-SAMHD1 antibody (ab192595) at 1/1000 dilution + Human fetal liver lysate at 10 µgSecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-SAMHD1 antibody (ab192595) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : Human fetal heart lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of stomach tissue labeling SAMHD1 with ab192595 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Both nucleus and weakly cytoplasm staining on Human adenocarcinoma of stomach tissue is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling SAMHD1 with ab192595 at 1/800 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Both nucleus and weakly cytoplasm staining on Human spleen tissue is observed. Counter stained with Hematoxylin.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling SAMHD1 with ab192595 at 1/200 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.