Anti-SAMHD1 antibody [1F9]
| Name | Anti-SAMHD1 antibody [1F9] |
|---|---|
| Supplier | Abcam |
| Catalog | ab117908 |
| Prices | $385.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 1F9 |
| Applications | IHC-P WB FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length Human SAMHD1 protein (NP_056289) produced in HEK293T cell |
| Description | Mouse Monoclonal |
| Gene | SAMHD1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody (ab117908) (Red), compared to a nonspecific negative control antibody (Blue).
![All lanes : Anti-SAMHD1 antibody [1F9] (ab117908) at 1/2000 dilutionLane 1 : HEK293T cells transfected with the pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with the pCMV6-ENTRY SAMHD1 cDNA Lysates/proteins at 5 µg per lane.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/26681_SAMHD1-Primary-antibodies-ab117908-2.jpg)
All lanes : Anti-SAMHD1 antibody [1F9] (ab117908) at 1/2000 dilutionLane 1 : HEK293T cells transfected with the pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with the pCMV6-ENTRY SAMHD1 cDNA Lysates/proteins at 5 µg per lane.
IHC image of SAMHD1 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab117908, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Interferon block to HIV-1 transduction in macrophages despite SAMHD1 degradation - Interferon block to HIV-1 transduction in macrophages despite SAMHD1 degradation
Dragin L, Nguyen LA, Lahouassa H, Sourisce A, Kim B, Ramirez BC, Margottin-Goguet F. Retrovirology. 2013 Mar 11;10:30.
SAMHD1 restricts herpes simplex virus 1 in macrophages by limiting DNA - SAMHD1 restricts herpes simplex virus 1 in macrophages by limiting DNA
Kim ET, White TE, Brandariz-Nunez A, Diaz-Griffero F, Weitzman MD. J Virol. 2013 Dec;87(23):12949-56.