All lanes : Anti-SART3 antibody (ab176822) at 0.02 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgdeveloped using the ECL technique
Detection of SART3 by Western Blot of Immunoprecipitate.abX176822 at 0.04µg/ml labeling SART3 in HeLa whole cell lysate immunoprecipitated using ab176822 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure times of 3seconds.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovarian carcinoma tissue labeling SART3 with ab176822 at 1 µg/ml.Detection: DAB staining.
Immunohistofluorescent analysis of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling SART3 with ab176822 at 0.5 µg/ml.Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1/100.