Anti-SART3 antibody (ab169302) at 1/500 dilution + Human pancreas lysate at 50 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique
All lanes : Anti-SART3 antibody (ab169302) at 1/500 dilutionLane 1 : SART3-transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique
Immunofluorescent analysis of HeLa cells labeling SART3 with purified version of ab169302 at 10µg/ml. Antibody purification with Protein A will be needed prior to use.