Anti-SATB1 antibody
| Name | Anti-SATB1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab49061 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ELISA IP WB IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human, Rat, Rabbit, Horse, Guinea Pig, Cat |
| Antigen | A region within synthetic peptide: DVAEYKEEEL LKDLEESVQD KNTNTLFSVK LEEELSVEGN TDINTDLKD, corresponding to C terminal amino acids 715-763 of Human SATB1 Run BLAST with Run BLAST with |
| Blocking Peptide | Human SATB1 peptide |
| Description | Rabbit Polyclonal |
| Gene | SATB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lane 1 : Anti-SATB1 antibody (ab49061) at 1.25 µg/mlLane 2 : Anti-SATB1 antibody (ab49061) at 2.5 µg/mlLane 3 : Anti-SATB1 antibody (ab49061) at 5 µg/mlLane 1 : Cell lysate prepared from human Jurkat cellsLane 2 : Cell lysate prepared from human Jurkat cellsLane 3 : Cell lysate prepared from human Jurkat cellsLysates/proteins at 25 µg per lane.
ab49061 staining SATB1 in HEK 293T cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed with formaldehyde, permeabilized using 0.1 % Triton X-100 for 5 minutes, blocked with 2% BSA for 1 hour at 25°C and then incubated with ab49061 at a 1/100 dilution for 3 hours at 25°C. The secondary used was an Oregon Green 488 conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.See Abreview
![Estrogen Related Receptor gamma was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Estrogen Related Receptor gamma and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab49061.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 100KDa: Estrogen Related Receptor gamma](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/27018_SATB1-Primary-antibodies-ab49061-43.jpg)
Estrogen Related Receptor gamma was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Estrogen Related Receptor gamma and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab49061.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 100KDa: Estrogen Related Receptor gamma
Product References
Upregulation of SATB1 is associated with prostate cancer aggressiveness and - Upregulation of SATB1 is associated with prostate cancer aggressiveness and
Shukla S, Sharma H, Abbas A, MacLennan GT, Fu P, Danielpour D, Gupta S. PLoS One. 2013;8(1):e53527.
Satb1 ablation alters temporal expression of immediate early genes and reduces - Satb1 ablation alters temporal expression of immediate early genes and reduces
Balamotis MA, Tamberg N, Woo YJ, Li J, Davy B, Kohwi-Shigematsu T, Kohwi Y. Mol Cell Biol. 2012 Jan;32(2):333-47.
SATB1 defines the developmental context for gene silencing by Xist in lymphoma - SATB1 defines the developmental context for gene silencing by Xist in lymphoma
Agrelo R, Souabni A, Novatchkova M, Haslinger C, Leeb M, Komnenovic V, Kishimoto H, Gresh L, Kohwi-Shigematsu T, Kenner L, Wutz A. Dev Cell. 2009 Apr;16(4):507-16.