Anti-SATB1 antibody [EPR3895]
| Name | Anti-SATB1 antibody [EPR3895] |
|---|---|
| Supplier | Abcam |
| Catalog | ab92307 |
| Prices | $403.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR3895 |
| Applications | WB IP IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human SATB1 aa 1-100 (N terminal) |
| Description | Rabbit Monoclonal |
| Gene | SATB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-SATB1 antibody [EPR3895] (ab92307) at 1/1000 dilution (unpurified)Lane 1 : Jurkat cell lysateLane 2 : Mouse thymus tissue lysateLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/27031_SATB1-Primary-antibodies-ab92307-1.jpg)
All lanes : Anti-SATB1 antibody [EPR3895] (ab92307) at 1/1000 dilution (unpurified)Lane 1 : Jurkat cell lysateLane 2 : Mouse thymus tissue lysateLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human tonisl tissue labelling SATB1 with unpurified ab92307 at a 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human colonic carcinoma tissue labelling SATB1 with unpurified ab92307 at a 1/50 dilution.
Unpurified ab92307 staining SATB1 in murine primary thymocytes (isolated from a 21 day old male mouse) by Immunocytochemistry/ Immunofluorescence. Cells were fixed in formaldehyde, permeabilized using 0.1% Triton X-100 for 5 minutes, blocked with 2% BSA for 1 hour at 25°C and then incubated with unpurified ab92307 at a 1/200 dilution for 3 hours at 25°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.See Abreview
Overlay histogram showing Jurkat cells stained with unpurified ab92307 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92307 (unpurified), 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Unpurified ab92307 immunoprecipitating SATB1 in Jurkat cell lysate.
Product References
Co-delivery of doxorubicin and SATB1 shRNA by thermosensitive magnetic cationic - Co-delivery of doxorubicin and SATB1 shRNA by thermosensitive magnetic cationic
Peng Z, Wang C, Fang E, Lu X, Wang G, Tong Q. PLoS One. 2014 Mar 27;9(3):e92924.
Expression of SATB1 promotes the growth and metastasis of colorectal cancer. - Expression of SATB1 promotes the growth and metastasis of colorectal cancer.
Zhang Y, Tian X, Ji H, Guan X, Xu W, Dong B, Zhao M, Wei M, Ye C, Sun Y, Yuan X, Yang C, Hao C. PLoS One. 2014 Jun 27;9(6):e100413.
Involvement of the SATB1/F-actin complex in chromatin reorganization during - Involvement of the SATB1/F-actin complex in chromatin reorganization during
Grzanka D, Gagat M, Izdebska M. Int J Mol Med. 2014 Jun;33(6):1441-50.
Satb1 regulates the self-renewal of hematopoietic stem cells by promoting - Satb1 regulates the self-renewal of hematopoietic stem cells by promoting
Will B, Vogler TO, Bartholdy B, Garrett-Bakelman F, Mayer J, Barreyro L, Pandolfi A, Todorova TI, Okoye-Okafor UC, Stanley RF, Bhagat TD, Verma A, Figueroa ME, Melnick A, Roth M, Steidl U. Nat Immunol. 2013 May;14(5):437-45.
Molecular correlates and prognostic significance of SATB1 expression in - Molecular correlates and prognostic significance of SATB1 expression in
Nodin B, Johannesson H, Wangefjord S, O'Connor DP, Lindquist KE, Uhlen M, Jirstrom K, Eberhard J. Diagn Pathol. 2012 Aug 30;7:115.
Immunohistochemistry profiles of breast ductal carcinoma: factor analysis of - Immunohistochemistry profiles of breast ductal carcinoma: factor analysis of
Laurinavicius A, Laurinaviciene A, Ostapenko V, Dasevicius D, Jarmalaite S, Lazutka J. Diagn Pathol. 2012 Mar 16;7:27.
Expression of the global regulator SATB1 is an independent factor of poor - Expression of the global regulator SATB1 is an independent factor of poor
Nodin B, Hedner C, Uhlen M, Jirstrom K. J Ovarian Res. 2012 Sep 19;5(1):24.