Anti-SATB2 antibody

• Supplier: Abcam
• Catalog: ab34735
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB EMSA IHC-F ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Mouse SATB2
• Description: Rabbit Polyclonal
• Gene: SATB2
• Conjugate: Unconjugated

Product images

Anti-SATB2 antibody (ab34735) at 1 µg/ml + Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) at 10 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.

ab34735 used in EMSA.EMSA demonstrating binding of SATB1 and SATB2 to an oligonucleotide containing a predicted SATB-binding element located 125 bp upstream of the Eomes transcription start site (-125). Labeled SATB probes were incubated with Rcho-1 TS cell nuclear lysates as indicated above the lanes. Lane 1, without any competitor oligonucleotide; lane 2, with unlabeled wild-type competitor; lane 3, with unlabeled mutant competitor; lane 4, with SATB1 antibody; lane 5, with SATB2 antibody (ab34735); lane 6, with a control antibody. B and C, Rcho-1 TS cells stably transfected with pcDNA3, pcDNA3-HA-Satb1, or pcDNA3-HA-Satb2 and maintained in stem culture conditions were used for ChIP assays. Antibodies to the HA tag (Anti-HA) or acetylated histone H3K9 (Anti-AcH3) were used in the analyses. IgG was used as a control for nonspecific immunoprecipitation. qPCR analyses were performed on immunoprecipitates using primers amplifying the segment (-172 to -63) including the SATB-binding eleme

ab34735 staining SATB2 in human osteosarcoma SAOS-2 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using PBS/ 0.25% Triton, blocked with 1% BSA for 1 hour at room temperature and then incubated with ab34735 at a 1/500 dilution for 1 hour. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/250 dilution.See Abreview

Product References

SATB homeobox proteins regulate trophoblast stem cell renewal and - SATB homeobox proteins regulate trophoblast stem cell renewal and

Asanoma K, Kubota K, Chakraborty D, Renaud SJ, Wake N, Fukushima K, Soares MJ, Rumi MA. J Biol Chem. 2012 Jan 13;287(3):2257-68.

A network connecting Runx2, SATB2, and the miR-23a~27a~24-2 cluster regulates the - A network connecting Runx2, SATB2, and the miR-23a~27a~24-2 cluster regulates the

Hassan MQ, Gordon JA, Beloti MM, Croce CM, van Wijnen AJ, Stein JL, Stein GS, Lian JB. Proc Natl Acad Sci U S A. 2010 Nov 16;107(46):19879-84. doi:

FIAT is co-expressed with its dimerization target ATF4 in early osteoblasts, but - FIAT is co-expressed with its dimerization target ATF4 in early osteoblasts, but

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Characterization of exosome-like vesicles released from human tracheobronchial - Characterization of exosome-like vesicles released from human tracheobronchial

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SATB1 defines the developmental context for gene silencing by Xist in lymphoma - SATB1 defines the developmental context for gene silencing by Xist in lymphoma

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The cell-intrinsic requirement of Sox6 for cortical interneuron development. - The cell-intrinsic requirement of Sox6 for cortical interneuron development.

Batista-Brito R, Rossignol E, Hjerling-Leffler J, Denaxa M, Wegner M, Lefebvre V, Pachnis V, Fishell G. Neuron. 2009 Aug 27;63(4):466-81.