Anti-SATB2 antibody [EPNCIR130A]

• Supplier: Abcam
• Catalog: ab92446
• Prices: $99.00, $397.00
• Sizes: 10 µl, 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPNCIR130A
• Applications: WB IHC-P ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human SATB2
• Description: Rabbit Monoclonal
• Gene: SATB2
• Conjugate: Unconjugated

Product images

Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution (purified) + SW1353 cell lysate at 20 µgSecondaryPeroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution (purified)Lane 1 : Saos-2 cell lysateLane 2 : Human fetal brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution (purified)Lane 1 : Mouse brain tissue lysateLane 2 : Rat brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryPeroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-SATB2 antibody [EPNCIR130A] (ab92446) at 1/1000 dilution (unpurified)Lane 1 : HT-1080 cell lysateLane 2 : SW1353 cell lysateLane 3 : MCF7 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling SATB2 with purified ab92446 at 1/150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Unpurified ab92446 staining SATB2 in mouse brain tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.3% Triton-X-100. Samples were then blocked with 5% serum for 1 hour 30 minutes at 20°C followed by incubation with the primary antibody at a 1/200 dilution for 36 hours. A biotin conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/200 dilution.See Abreview

Unpurified ab92446 staining SATB2 in E18 Mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton-X 100 and blocked with 3% BSA for 30 minutes at 25°C. The sample was incubated with primary antibody (1/500 in TBS with 0.1% Triton-X 100 + 3% Goat serum) at 4°C for 12 hours. An Alexa Fluor® 546-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.See Abreview

Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling SATB2 (green) with purified ab92446 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/100) and secondary antibody ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

Product References

Cortical neurogenesis in the absence of centrioles. - Cortical neurogenesis in the absence of centrioles.

Insolera R, Bazzi H, Shao W, Anderson KV, Shi SH. Nat Neurosci. 2014 Nov;17(11):1528-35.