Anti-SATB2 antibody [SATBA4B10]

• Supplier: Abcam
• Catalog: ab51502
• Prices: $401.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: SATBA4B10
• Applications: IP ICC/IF ICC/IF IHC-F WB IHC-F IHC-P
• Species Reactivities: Mouse, Rat, Human, Zebrafish
• Antigen: Recombinant fragment C-terminal
• Description: Mouse Monoclonal
• Gene: SATB2
• Conjugate: Unconjugated

Product images

Anti-SATB2 antibody [SATBA4B10] (ab51502) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µgSecondaryAnti Mouse IgG antibody at 1/2500 dilution

Immunoprecipitation using ab51502 at 1:100 dilution. Sample :NIH3T3 whole cell lysate 25µg. Secondary used at 1:2500.

ab51502 staining SATB2 in HeLa cells by Immunocytochemistry 1:200.

ab51502 staining cultured rat cortical neuron cells by ICC/IF.  Cells were PFA fixed, permeabilized in 0.1% triton and blocked with 10% BSA for 1 hour at 22°C.  The sample was then incubated with ab51502, diluted 1/25 for 2 hours at 22°C prior to incubation with a Cy3® conjugated donkey anti-mouse antibody, diluted 1/400.  In the image, red is SATB2 and green is F-actinSee Abreview

ab51502 staining SATB2 in mouse brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to mouse IgG (1/1000) was used as secondary antibody. See Abreview

ab51502 staining SATB2 in human fetal cortex tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer pH 6.0. Samples were then blocked with 10% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/100 dilution for 24 hours at 4°C. A Cy3®-conjugated horse anti-mouse polyclonal was used as secondary antibody at a 1/200 dilution. Counter-stained with DAPI (blue).See Abreview

ab51502 staining SATB2 (red) in a mouse cortex e17.5 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 3% Donkey serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/10) for 90 minutes at 22°C. An Alexa Fluor®555-conjugated Donkey anti-mouse polyclonal (1/200) was used as the secondary antibody. Green: CTIP2 (ab18465) staining Blue: TBR1 (ab31940) stainingSee Abreview

Product References

Gli3 controls corpus callosum formation by positioning midline guideposts during - Gli3 controls corpus callosum formation by positioning midline guideposts during

Magnani D, Hasenpusch-Theil K, Benadiba C, Yu T, Basson MA, Price DJ, Lebrand C, Theil T. Cereb Cortex. 2014 Jan;24(1):186-98.

Alteration of rat fetal cerebral cortex development after prenatal exposure to - Alteration of rat fetal cerebral cortex development after prenatal exposure to

Naveau E, Pinson A, Gerard A, Nguyen L, Charlier C, Thome JP, Zoeller RT, Bourguignon JP, Parent AS. PLoS One. 2014 Mar 18;9(3):e91903.

Agenesis of the Corpus Callosum Due to Defective Glial Wedge Formation in Lhx2 - Agenesis of the Corpus Callosum Due to Defective Glial Wedge Formation in Lhx2

Chinn GA, Hirokawa KE, Chuang TM, Urbina C, Patel F, Fong J, Funatsu N, Monuki ES. Cereb Cortex. 2014 Apr 29.

Transcallosal Projections Require Glycoprotein M6-Dependent Neurite Growth and - Transcallosal Projections Require Glycoprotein M6-Dependent Neurite Growth and

Mita S, de Monasterio-Schrader P, Funfschilling U, Kawasaki T, Mizuno H, Iwasato T, Nave KA, Werner HB, Hirata T. Cereb Cortex. 2014 Jun 10. pii: bhu129.

Cortical and Clonal Contribution of Tbr2 Expressing Progenitors in the Developing - Cortical and Clonal Contribution of Tbr2 Expressing Progenitors in the Developing

Vasistha NA, Garcia-Moreno F, Arora S, Cheung AF, Arnold SJ, Robertson EJ, Molnar Z. Cereb Cortex. 2014 Jun 13. pii: bhu125.

Satb2 Regulates the Differentiation of Both Callosal and Subcerebral Projection - Satb2 Regulates the Differentiation of Both Callosal and Subcerebral Projection

Leone DP, Heavner WE, Ferenczi EA, Dobreva G, Huguenard JR, Grosschedl R, McConnell SK. Cereb Cortex. 2014 Jul 17. pii: bhu156.

Characterization of excitatory and inhibitory neuron activation in the mouse - Characterization of excitatory and inhibitory neuron activation in the mouse

Gaykema RP, Nguyen XM, Boehret JM, Lambeth PS, Joy-Gaba J, Warthen DM, Scott MM. Front Neuroanat. 2014 Jul 1;8:60.

The Snail transcription factor regulates the numbers of neural precursor cells - The Snail transcription factor regulates the numbers of neural precursor cells

Zander MA, Cancino GI, Gridley T, Kaplan DR, Miller FD. PLoS One. 2014 Aug 19;9(8):e104767.

Primary cilia are required in a unique subpopulation of neural progenitors. - Primary cilia are required in a unique subpopulation of neural progenitors.

Tong CK, Han YG, Shah JK, Obernier K, Guinto CD, Alvarez-Buylla A. Proc Natl Acad Sci U S A. 2014 Aug 26;111(34):12438-43. doi:

The serotonin 6 receptor controls neuronal migration during corticogenesis via a - The serotonin 6 receptor controls neuronal migration during corticogenesis via a

Jacobshagen M, Niquille M, Chaumont-Dubel S, Marin P, Dayer A. Development. 2014 Sep;141(17):3370-7.