Anti-SCF antibody [EP665Y]
| Name | Anti-SCF antibody [EP665Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab52603 |
| Prices | $398.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP665Y |
| Applications | WB FC IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | A synthetic peptide corresponding to residues near the N-terminus of human SCF |
| Description | Rabbit Monoclonal |
| Gene | KITLG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-SCF antibody [EP665Y] (ab52603) at 1/10000 dilution + recombinant protein (5ng)SecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/27305_ab52603wb.jpg)
Anti-SCF antibody [EP665Y] (ab52603) at 1/10000 dilution + recombinant protein (5ng)SecondaryHRP-labelled goat anti-rabbit at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue using ab52603 at a dilution of 1/100-1/250.
Overlay histogram showing MCF-7 cells stained with ab52603 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52603, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
Cardiac-specific overexpression of human stem cell factor promotes epicardial - Cardiac-specific overexpression of human stem cell factor promotes epicardial
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Single-cell clones of liver cancer stem cells have the potential of - Single-cell clones of liver cancer stem cells have the potential of
Liu H, Zhang W, Jia Y, Yu Q, Grau GE, Peng L, Ran Y, Yang Z, Deng H, Lou J. Cell Death Dis. 2013 Oct 17;4:e857.
Meiotic activity in orthotopic xenografts derived from human postpubertal - Meiotic activity in orthotopic xenografts derived from human postpubertal
Van Saen D, Goossens E, Bourgain C, Ferster A, Tournaye H. Hum Reprod. 2011 Feb;26(2):282-93.