All lanes : Anti-SCIN antibody [EPR16310] - C-terminal (ab199723) at 1/10000 dilutionLane 1 : Human fetal kidney lysateLane 2 : HEK293 (Human embryonic kidney) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
All lanes : Anti-SCIN antibody [EPR16310] - C-terminal (ab199723) at 1/1000 dilutionLane 1 : Mouse kidney lysateLane 2 : Mouse spleen lysateLane 3 : Rat kidney lysateLane 4 : Rat spleen lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-SCIN antibody [EPR16310] - C-terminal (ab199723) at 1/1000 dilutionLane 1 : C6 (rat glial tumor cells) whole cell lysateLane 2 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysateLane 3 : NIH/3T3 (mouse embryo fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney tissue labeling SCIN with ab199723 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human clear cell carcinoma of kidney tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SCIN with ab199723 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human pancreas tissue is observed. Counter stained with Hematoxylin.Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).