ICC/IF image of ab107370 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab107370 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-SCN1B antibody (ab107370) at 1/100 dilution + Mouse Neuro-2a cell line lysates at 35 µg
ab107370 at 1/50 dilution staining SCN1B in formalin-fixed and paraffin-embedded Human brain tissue by Immunohistochemistry, followed by peroxidase conjugation of the secondary antibody and DAB detection.