All lanes : Anti-SCN4B antibody (ab80539) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Rat) Tissue Lysate Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate Lane 4 : Spinal Cord (Human) Tissue Lysate - adult normal tissue (ab29188)Lane 5 : Spinal Cord (Rat) Tissue Lysate Lane 6 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lysates/proteins at 10 µg/ml per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 37 kDa (why is the actual band size different from the predicted?)Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.Exposure time : 8 minutes
ICC/IF image of ab80539 stained PC12 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80539, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.