Anti-SCRIBBLE antibody
| Name | Anti-SCRIBBLE antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab115240 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB |
| Species Reactivities | Human, Rat, Horse, Chimpanzee, Monkey, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human SCRIBBLE |
| Description | Rabbit Polyclonal |
| Gene | SCRIB |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-SCRIBBLE antibody (ab115240) at 1 µg/ml + Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab115240 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab115240 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.