SCRIB

Gene Symbol SCRIB
Entrez Gene 23513
Alt Symbol CRIB1, SCRB1, SCRIB1, Vartul
Species Human
Gene Type protein-coding
Description scribbled planar cell polarity protein
Other Description protein scribble homolog|scribbled homolog
Swissprots Q14160 Q8WWV8 Q6P496 Q96C69 Q7Z5D1 Q96GG1
Accessions EAW82196 EAW82197 EAW82198 Q14160 AF240677 AAP88017 AF271734 AAP88018 AF318352 AAL55859 AI829609 AY062238 AAL38976 BC009490 AAH09490 BC014632 AAH14632 BC026045 BC036905 BC044627 AAH44627 BC063590 BC146321 AAI46322 D63481 BAA09768 NM_015356 NP_056171 NM_182706 NP_874365
Function Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity. {ECO:0000269|PubMed:15182672, ECO:0000269|PubMed:15775968, ECO:0000269|PubMed:16344308, ECO:0000269|PubMed:16965391, ECO:0000269|PubMed:18641685, ECO:0000269|PubMed:18716323, ECO:0000269|PubMed:19041750}.
Subcellular Location Cell membrane; Peripheral membrane protein. Cell junction, adherens junction. Cell projection, lamellipodium. Cytoplasm. Note=Targeting to cell-cell junctions which is CDH1- dependent is required for the pro-apoptotic activity. Localizes to neuronal post- and pre-synaptic regions.
Tissue Specificity Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level). {ECO:0000269|PubMed:15806148}.
Top Pathways Viral carcinogenesis, Hippo signaling pathway

Phospho-Scribble (Ser1220) (D8A2) Rabbit mAb - 12316 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8A2
Western blot analysis of extracts from 293T, PANC-1, and HCT 116 cells, untreated (-) or λ phosphatase-treated (+), using Phospho-Scribble (Ser1220) (D8A2) Rabbit mAb (upper), Scribble Antibody #4475 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Scribble Antibody - 4475 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Scribble Antibody.

Scrib (D-8) - sc-55532 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Scrib (D-8): sc-55532. Western blot analysis of Scrib expression in HeLa (A) and Jurkat (B) whole cell lysates.

Scrib (D-2) - sc-374139 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Scrib (D-2): sc-374139. Western blot analysis of Scrib expression in Jurkat whole cell lysate.

Scrib (C-6) - sc-55543 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Scrib (C-6): sc-55543. Western blot analysis of Scrib expression in HeLa (A) and Jurkat (B) whole cell lysates.

Scrib (H-300) - sc-28737 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Scrib (H-300): sc-28737. Immunofluorescence staining of normal mouse kidney frozen section showing cytoplasmic staining.

Scrib (C-20) - sc-11049 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Scrib siRNA (h): sc-36466. Western blot analysis of Scrib expression in non-transfected control (A) and Scrib siRNA transfected (B) HeLa cells. Blot probed with Scrib (C-20): sc-11049. α-actinin (H-2): sc-17829 used as specificity and loading control.

Scrib (K-21) - sc-11048 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Scrib (K-21): sc-11048. Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse kidney tissue showing tight junction localization.

Anti-SCRIBBLE antibody - ab115240 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-SCRIBBLE antibody (ab115240) at 1 µg/ml + Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-SCRIBBLE antibody - ab36708 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab36708 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36708, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SCRIBBLE antibody - ab154067 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-SCRIBBLE antibody (ab154067) at 1/1000 dilution + HepG2 whole cell lysates at 30 µg

Anti-SCRIBBLE antibody [EPR4140(2)] - ab125080 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4140(2)
All lanes : Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : MCF7 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-SCRIBBLE antibody - N-terminal - ab138763 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-SCRIBBLE antibody - N-terminal (ab138763) at 1/100 dilution + Human placenta tissue lysate at 35 µg

Anti-SCRIB / Scribble Antibody - LS-C132040 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SCRIB / Scribble Antibody (C-Terminus) - LS-C209983 from LifeSpan Bioscience

Prices $405.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-SCRIB / Scribble Antibody (aa381-410, APC) - LS-C285257 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SCRIB / Scribble Antibody (Internal) - LS-C209984 from LifeSpan Bioscience

Prices $295.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-SCRIB / Scribble Antibody (aa381-410, AP) - LS-C285254 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SCRIB / Scribble Antibody (aa381-410, Biotin) - LS-C285258 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SCRIB / Scribble Antibody (aa381-410, FITC) - LS-C285259 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SCRIB / Scribble Antibody (aa381-410, Azide-free, HRP) - LS-C285260 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SCRIB / Scribble Antibody (aa381-410) - LS-C330097 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SCRIB / Scribble Antibody (aa381-410, PE) - LS-C285261 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SCRIB / Scribble Antibody (aa381-410) - LS-C158928 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
SCRIB Antibody western blot of human placenta tissue lysates (35 ug/lane). The SCRIB antibody detected the SCRIB protein (arrow).

Anti-SCRIB / Scribble Antibody (C-Terminus, clone EPR4140(2)) - LS-C138330 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4140(2)
Western blot analysis on (A) 293T, (B) HeLa, and (C) MCF-7 cell lysates using anti-Scribble antibody.
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