Anti-SDF1 beta antibody
| Name | Anti-SDF1 beta antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab18919 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP IHC-P IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Recombinant full length protein corresponding to Mouse SDF1 beta |
| Description | Rabbit Polyclonal |
| Gene | CXCL12 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab18919 (2µg/ml) staining SDF1 beta in human gallbladder using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic/secretory compartments within the smooth muscle fibres.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.