CXCL12

Gene Symbol CXCL12
Entrez Gene 6387
Alt Symbol IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1
Species Human
Gene Type protein-coding
Description chemokine (C-X-C motif) ligand 12
Other Description intercrine reduced in hepatomas|pre-B cell growth-stimulating factor|stromal cell-derived factor 1
Swissprots H7BYN8 E7EVL0 Q6ICW0 P48061 Q2L985 Q5IT36 Q2L988 Q9H554 Q2L986 B2R4G0
Accessions AAV49999 CAJ21099 CAJ21100 EAW86618 EAW86619 EAW86620 EAW86621 P48061 AI092156 AJ227905 AK090482 BAC03463 AK124641 AK292628 BAF85317 AK311814 BAG34757 AL713778 CAD28539 AU120056 AW014388 AY644456 AAT76437 AY874118 AAW82036 BC031072 BC039893 AAH39893 BX647204 CD172464 CR450283 CAG29279 DA266752 DQ345517 ABC69270 DQ345518 ABC69271 DQ345519 ABC69272 DQ345520 ABC69273 L36033 AAB39332 L36034 AAB39333 U16752 AAA97434 NM_000609 NP_000600 NM_001033886 NP_001029058 NM_001178134 NP_001171605 NM_001277990 NP_001264919 NM_199168 NP_954637
Function Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1- alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3- 67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-me
Subcellular Location Secreted.
Tissue Specificity Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. {ECO:0000269|PubMed:16626895}.
Top Pathways Cytokine-cytokine receptor interaction, Axon guidance, Leukocyte transendothelial migration, Intestinal immune network for IgA production, Chemokine signaling pathway

SDF1 Antibody - 3740 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of recombinant human SDF1 protein using SDF1 Antibody.

SDF1 (D32F9) Rabbit mAb - 3530 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D32F9
Western blot analysis of recombinant human SDF1 protein using SDF1 (D32F9) Rabbit mAb.

SDF-1 (P-159X) - sc-74271 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal

SDF-1 (FL-93) - sc-28876 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SDF-1 (FL-93): sc-28876. Western blot analysis of SDF-1 expression in RAW 264.7 whole cell lysate.

SDF-1 (C-19) - sc-6193 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SDF-1 (V-13) - sc-54144 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SDF-1 (A-24) - sc-133989 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Rabbit
Clonality Polyclonal
SDF-1 (A-24): sc-133989. Western blot analysis of SDF-1 expression in human fetal lung tissue extract.

Anti-SDF1 alpha antibody - ab9797 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab9797 staining SDF1 alpha in human colorectal adenocarcinoma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.5 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

Anti-SDF1 alpha antibody - ab25117 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
+ Recombinant mouse SDF1 alpha

Anti-SDF1 alpha antibody (Biotin) - ab84277 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-SDF1 alpha antibody - Azide free - ab175646 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SDF1 alpha antibody - Azide free - ab175622 from Abcam

Prices $0.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal

Anti-SDF1 antibody - ab181018 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SDF1 antibody (ab181018) at 1 µg/mlLane 1 : HeLa cell lysateLane 2 : HeLa cell lysate with blocking peptideLysates/proteins at 15 µg per lane.

Anti-SDF1 antibody - ab10395 from Abcam

Prices $370.00
Sizes 25 µg
Host Goat
Clonality Polyclonal

Anti-SDF1 antibody [EPR1216] - ab155090 from Abcam

Prices $378.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1216
Anti-SDF1 antibody [EPR1216] (ab155090) at 1/10000 dilution + Human CXCL12 recombinant protein at 0.01 µgSecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-SDF1 antibody [MM0211-9N26] - ab89321 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone MM0211-9N26

Anti-SDF1 antibody - C-terminal - ab168825 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-SDF1 antibody - C-terminal (ab168825) at 1/100 dilution + NCI-H292 cell line lysates at 35 µg

Anti-SDF1 beta antibody - ab18919 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab18919 (2µg/ml) staining SDF1 beta in human gallbladder using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cytoplasmic/secretory compartments within the smooth muscle fibres.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-SDF1 beta antibody - ab9799 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-SDF1 / CXCL12 Antibody IHC-plus™ - LS-B7489 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-CXCL12 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7489 concentration 2.5 ug/ml.

Anti-SDF1 / CXCL12 Antibody (C-Terminus) IHC-plus™ - LS-B943 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-CXCL12 antibody IHC of human skin, fibroblasts. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B943 concentration 5 ug/ml.

Anti-SDF-1 / CXCL12 Antibody - LS-C47461 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-SDF1 / CXCL12 Antibody - LS-C48888 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SDF1 / CXCL12 Antibody (Biotin) - LS-C86690 from LifeSpan Bioscience

Prices $405.00
Sizes 25 µg
Host Goat
Clonality Polyclonal

Anti-SDF1 / CXCL12 Antibody (AP) - LS-C87119 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
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