Anti-SEC22L1 antibody [EPR12335]

• Supplier: Abcam
• Catalog: ab181076
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR12335
• Applications: WB IHC-P ICC/IF ICC/IF IP FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SEC22L1 aa 1-100 (N terminal)
• Description: Rabbit Monoclonal
• Gene: SEC22B
• Conjugate: Unconjugated

Product images

Anti-SEC22L1 antibody [EPR12335] (ab181076) at 1/2000 dilution + SH-SY5Y cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling SEC22L1 with ab181076 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Western blot analysis of immunoprecipitation pellet from HeLa cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181076 at 1/80 dilution.Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. 

Flow cytometric analysis of SH-SY5Y cells (2% paraformaldehyde-fixed) labeling SEC22L1 with ab181076 at 1/110 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SEC22L1 with ab181076 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling SEC22L1 with ab181076 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Anti-SEC22L1 antibody [EPR12335] (ab181076) at 1/10000 dilution + MCF7 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution