Anti-Secretory Component Glycoprotein antibody [SC-05]

• Supplier: Abcam
• Catalog: ab3924
• Prices: $390.00
• Sizes: 100 µg
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: SC-05
• Applications: IHC-F IP WB IHC-P
• Species Reactivities: Human
• Antigen: Full length native protein (affinity-purified secretory component) (Human colostrum)
• Description: Mouse Monoclonal
• Gene: ECM1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Secretory Component Glycoprotein antibody [SC-05] (ab3924) at 1 µg/mlLane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 2 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ab3924 staining Secretory Component Glycoprotein in human normal breast tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in methanol, blocked using 10% serum for 5 minutes at 25°C and then incubated with ab3924 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.The images show stainings of normal breast glands from two different patients. The antibody mostly stains the apical/luminal region of luminal epithelial cells, but the intensity varies from patient to patient.See Abreview

Immunohistochemical analysis of Human breast cancer tissue, staining Secretory Component Glycoprotein with ab3924. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6). Tissue was blocked with 10% normal goat serum before incubating with primary antibody (1/50) for 2 hours at room temperature. Staining was primarily in the cytoplasm.

Product References

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Keratin 19 expression in the adult and developing human mammary gland. - Keratin 19 expression in the adult and developing human mammary gland.

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Secretory component in differentiating normal epithelium, benign lesions and - Secretory component in differentiating normal epithelium, benign lesions and

Bartek J, Tlaskalova-Hogenova H, Staskova Z, Simeckova J, Vojtesek B, Rejthar A, Kovarik J, Bartkova J. Histochemistry. 1989;91(3):235-44.

Rapid selection of cultured cells with increased expression of a membrane marker - Rapid selection of cultured cells with increased expression of a membrane marker

Kvale D, Bartek J, Sollid LM, Brandtzaeg P. Int J Cancer. 1988 Oct 15;42(4):638-41.