Anti-Securin antibody [DCS-280]

• Supplier: Abcam
• Catalog: ab3305
• Prices: $401.00
• Sizes: 500 µl
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: DCS-280
• Applications: ICC/IF ICC/IF WB IHC-P IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Human recombinant full length Pds 1 protein
• Description: Mouse Monoclonal
• Gene: PTTG1
• Conjugate: Unconjugated

Product images

ab3305 (1/200) staining Securin in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde (4%, 10 minutes), permeabilised (0.5% Triton X100 in PBS) and counterstained with DAPI in order to highlight the nucleus (red). Please refer to Abreview for further experimental details.See Abreview

ab3305 (1µg/ml) staining Securin in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and nuclear staining of cells of the germinal centre.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-Securin antibody [DCS-280] (ab3305) at 2 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 4 minutes

All lanes : Anti-Securin antibody [DCS-280] (ab3305) at 1/1000 dilutionLane 1 : AtT20 (mouse pituitary) cell lysateLane 2 : AtT20 (mouse pituitary) cell lysateLane 3 : AtT20 (mouse pituitary) cell lysateLane 4 : AtT20 (mouse pituitary) cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated Sheep anti-Mouse polyclonal at 1/1000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 27 kDa (why is the actual band size different from the predicted?)Exposure time : 10 secondsThis image is courtesy of an Anonymous Abreview.See Abreview

Anti-Securin antibody [DCS-280] (ab3305) at 1/1000 dilution + HeLa whole cell lysate at 50 µgSecondaryHRP-conjugated goat anti-mouse polyclonal IgG at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 20 secondsThis image is courtesy of an anonymous AbreviewSee Abreview

Product References

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