PTTG1

Gene Symbol PTTG1
Entrez Gene 9232
Alt Symbol EAP1, HPTTG, PTTG, TUTR1
Species Human
Gene Type protein-coding
Description pituitary tumor-transforming 1
Other Description ESP1-associated protein 1|pituitary tumor-transforming gene 1 protein|securin|tumor-transforming protein 1
Swissprots O95997
Accessions AAF06995 BAH69081 BAH69082 CAY55983 EAW61551 EAW61552 EAW61553 O95997 AF062649 AAC69752 AF075242 AAD19335 AF095287 AAC64409 AJ223953 CAA11683 AK312209 BAG35142 BC026003 BC101834 AAI01835 BC108894 AAI08895 BC108895 AAI08896 BC112071 AAI12072 BC113842 AAI13843 BC113896 AAI13897 BC128193 AAI28194 BC128194 AAI28195 BI827526 BM990391 BU182149 CR457135 CAG33416 CR541685 CAG46486 HY006026 NM_001282382 NP_001269311 NM_001282383 NP_001269312 NM_004219 NP_004210
Function Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation. {ECO:0000269|PubMed:10411507, ECO:0000269|PubMed:11238996, ECO:0000269|PubMed:11371342, ECO:0000269|PubMed:1
Subcellular Location Cytoplasm. Nucleus.
Tissue Specificity Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer. {ECO:0000269|PubMed:9811450}.
Top Pathways Cell cycle, Oocyte meiosis, HTLV-I infection

Securin (D2B6O) Rabbit mAb - 13445 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2B6O
Western blot analysis of extracts from HT-29 cells, untreated (-) or treated with thymidine (2 mM, 16 hr) followed by Nocodazole #2190 (10 nM, 24 hr; +), using Securin (D2B6O) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

PTTG (C-2) - sc-398471 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PTTG (C-2): sc-398471. Western blot analysis of PTTG expression in HCT-116 whole cell lysate.

PTTG (G-9) - sc-376454 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PTTG (G-9): sc-376454. Western blot analysis of PTTG expression in Jurkat whole cell lysate.

PTTG (D-17) - sc-5837 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PTTG (D-17): sc-5837. Western blot analysis of human recombinant PTTG fusion protein (A) and Peroxin 6 expression in Jurkat (B) and MOLT-4 (C) whole cell lysates.

PTTG (C-18) - sc-5839 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PTTG (C-18): sc-5839. Western blot analysis of PTTG expression in MOLT-4 whole cell lysate.

PTTG (M-16) - sc-5843 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PTTG (N-16) - sc-5836 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PTTG (N-16): sc-5836. Western blot analysis of human recombinant PTTG fusion protein (A) and PTTG expression in MOLT-4 whole cell lysate (B).

PTTG (S-19) - sc-67817 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PTTG (S-19): sc-67817. Western blot analysis of PTTG expression in Jurkat (A) and MOLT-4 (B) whole cell lysates.

PTTG (H-160) - sc-22772 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PTTG (H-160): sc-22772. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in seminiferous ducts and Leydig cells.

PTTG (DCS-280) - sc-56207 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PTTG (DCS-280): sc-56207. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic and membrane staining of cells in seminiferous ducts and cytoplasmic staining of Leydig cells.

PTTG (SPM210) - sc-56461 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PTTG (SPM210): sc-56461. Western blot analysis of PTTG expression in Jurkat whole cell lysate.

PTTG (3F313) - sc-71947 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PTTG (3F313): sc-71947. Western blot analysis of PTTG expression in non-transfected: sc-117752 (A) and mouse PTTG transfected: sc-125874 (B) 293T whole cell lysates.

Anti-Securin antibody - ab26273 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
HeLa cells stained with ab26273 (1/100 dilution). A clear nuclear signal in interphase HeLa cells can be seen.

Anti-Securin antibody - ab94689 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-Securin antibody (ab94689) at 1 µg/ml + COLO205 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-Securin antibody - ab64930 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Securin antibody (ab64930) at 1/500 dilutionLane 1 : Extracts from 293 cellsLane 2 : Extracts from Jurkat cellsLane 3 : Extracts from HepG2 cellsLane 4 : Extracts from HepG2 cells with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-Securin antibody - ab126170 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-Securin antibody (ab126170) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

Anti-Securin antibody - ab118567 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Human Testis (formalin-fixed, paraffin-embedded) stained with Securin antibody ab118567 at 5 µg/ml.

Anti-Securin antibody [DCS-280] - ab3305 from Abcam

Prices $401.00
Sizes 500 µl
Host Mouse
Clonality Monoclonal
Clone DCS-280
ab3305 (1/200) staining Securin in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde (4%, 10 minutes), permeabilised (0.5% Triton X100 in PBS) and counterstained with DAPI in order to highlight the nucleus (red). Please refer to Abreview for further experimental details.See Abreview

Anti-Securin antibody [DCS-280] - ab94815 from Abcam

Prices $376.00
Sizes 250 µl
Host Mouse
Clonality Monoclonal
Clone DCS-280
ab94815, at 1/100 dilution, staining formalin-fixed, paraffin-embedded Human tonsil by Immunohistochemistry using peroxidase-conjugate and AEC chromogen. Note nuclear staining of lympocytes in germinal center.

Anti-Securin antibody [DCS-280] - ab80569 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone DCS-280
Formalin fixed, paraffin embedded human tonsil tissue stained with anti Securin (an alternative form of ab80569, containing azide and BSA) at 2 µg/ml using peroxidase conjugate and AEC chromogen. Note nuclear staining of lympocytes in germinal center.

Anti-Securin antibody [EPR3240] - ab79546 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3240
All lanes : Anti-Securin antibody [EPR3240] (ab79546) at 1/20000 dilutionLane 1 : Daudi cell lysateLane 2 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-Securin antibody [EPR3240] (Alexa Fluor® 488) - ab199238 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3240
ab199238 staining Securin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199238 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Securin antibody [EPR3240] (Alexa Fluor® 647) - ab199496 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR3240
ab199496 staining Securin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199496 at a 1/150 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 4% formaldehyde (10 min).

Anti-Securin antibody [SPM210], prediluted - ab54187 from Abcam

Host Mouse
Clonality Monoclonal
Clone SPM210
Ab54187 staining human Securin in human tonsil tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue (tissue sections boiled in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min)

Anti-Securin / PTTG1 Antibody (N-Terminus) - LS-C9840 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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