Anti-Seladin 1 antibody
| Name | Anti-Seladin 1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab40490 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB |
| Species Reactivities | Mouse, Human, Rat, Bovine |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human Seladin 1 |
| Description | Rabbit Polyclonal |
| Gene | DHCR24 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Seladin 1 antibody (ab40490) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab40490 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab40490, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.
Product References
Crosstalk between thyroid hormone receptor and liver X receptor in the regulation - Crosstalk between thyroid hormone receptor and liver X receptor in the regulation
Ishida E, Hashimoto K, Okada S, Satoh T, Yamada M, Mori M. PLoS One. 2013;8(1):e54901.
Thyroid hormone receptor and liver X receptor competitively up-regulate human - Thyroid hormone receptor and liver X receptor competitively up-regulate human
Ishida E, Hashimoto K, Okada S, Satoh T, Yamada M, Mori M. Biochem Biophys Res Commun. 2013 Mar 15;432(3):513-8. doi: