All lanes : Anti-SELS antibody (ab190247) at 0.4 µg/mlLane 1 : HeLa whole cell lysate in NETN bufferLane 2 : HeLa whole cell lysate in RIPA bufferLane 3 : 293T whole cell lysate in NETN bufferLane 4 : Jurkat whole cell lysate in RIPA bufferLysates/proteins at 50 µg per lane.developed using the ECL technique
Immunoprecipitation analysis of HeLa cell lysate (in NETN buffer, 0.5 or 1.0 mg per IP reaction; 20% of IP loaded) labeling SELS using ab190247 at 6 μg per reaction (lane 1). a Control IgG was used in lane 2. For blotting immunoprecipitated SELS, ab190247 was used at 1 μg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.