Anti-Semaphorin 3A antibody
| Name | Anti-Semaphorin 3A antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab23393 |
| Prices | $401.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IHC-F ICC/IF ICC/IF IHC-F WB ELISA |
| Species Reactivities | Mouse, Rat, Chicken, Human, Xenopus, Zebrafish, Fish |
| Antigen | Synthetic peptide (coupled to carrier protein): RDGPNYQWVPYQGR , corresponding to amino acids 359-372 of Human Semaphorin 3A (Peptide available as ab42768) |
| Blocking Peptide | Active human Semaphorin 3A peptide |
| Description | Rabbit Polyclonal |
| Gene | SEMA3A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lanes 1 - 2 : Anti-Semaphorin 3A antibody (ab23393) at 1/1000 dilutionLanes 3 - 4 : Anti-Semaphorin 3A antibody (ab25999)Lane 1 : Neonatal rat brain Lane 2 : Human recombinant Sema3A/Fc chimera (95/125 kDa) Lane 3 : Neonatal rat brainLane 4 : Human recombinant Sema3A/Fc chimera (95/125 kDa)
Ab23393 staining human normal ileum. Staining is localized to the cytoplasm.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Anti-Semaphorin 3A antibody (ab23393) at 1/1000 dilution + Lysate prepared from Xenopus laevis brain tissue section at 15 µgSecondaryHRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab23393 staining Semaphorin 3A in 16 µm thick sections of Apteronotus leptorhynchus brain, spinal cord by Immunohistochemistry (Frozen sections).Tissue was fixed in 2% paraformaldehyde, permeabilized using 0.3% Triton X-100, then blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at 24°C and then incubated with ab23393 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.See Abreview
Immunohistochemical analysis of murine developing cerebral cortices, staining Semaphorin 3A with ab23393.
Product References
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