Anti-Septin 2 antibody
| Name | Anti-Septin 2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab88657 |
| Prices | $385.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Human |
| Antigen | Full length Human Septin 2 (NP_001008491, amino acids 1-361) |
| Description | Mouse Polyclonal |
| Gene | SEPT2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-Septin 2 antibody (ab88657) at 1 µg/ml + human spleen tissue lysate at 50 µg
All lanes : Anti-Septin 2 antibody (ab88657) at 1 µg/mlLane 1 : SEPT2 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.
ab88657 at 10µg/ml staining SEPT2 in HeLa cells by Immunofluorescence.
IHC image of Septin 2 staining in Human breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88657, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.