Anti-Septin 2 antibody [EPR12122]

• Supplier: Abcam
• Catalog: ab187654
• Prices: $384.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR12122
• Applications: ICC/IF ICC/IF WB IHC-P FC
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Septin 2 aa 1-100
• Description: Rabbit Monoclonal
• Gene: SEPT2
• Conjugate: Unconjugated

Product images

Immunofluorescence analysis of MCF7 cells labeling Septin 2 using ab187654 at 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.

All lanes : Anti-Septin 2 antibody [EPR12122] (ab187654) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : MCF7 cell lysateLane 3 : U-87 MG cell lysateLane 4 : Human fetal brain lysateLysates/proteins at 20 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

All lanes : Anti-Septin 2 antibody [EPR12122] (ab187654) at 1/1000 dilutionLane 1 : mouse Raw 264.7 cell lysateLane 2 : rat PC12 cell lysateLane 3 : mouse NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilutiondeveloped using the ECL technique

Immunohistochemical analysis of paraffin-embedded, Human breast carcinoma tissue labeling Septin 2 with ab187654 at a 1/1000 dilution. Counter stained with hematoxylin.

Immunofluorescence analysis of, paraformaldehyde-fixed, MCF-7 cell line labeling Septin 2 with ab187654 at a 1/100 dilution. As secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) was used at a 1/200 (left panel). In blue DAPI staining (right panel).

Flow cytometry analysis of paraformaldehyde-fixed Jurkat cells labeling Septin 2 with ab187654 at a 1/120 dilution and secondary antibody FITC goat anti-rabbit IgG (red) at a 1/150 dilution, or negative control rabbit IgG (green).