All lanes : Anti-Septin 2 antibody [EPR12123] (ab179436) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : U87-MG lysateLane 3 : Jurkat lysateLane 4 : MCF-7 lysateLysates/proteins at 10 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Septin 2 with ab179436 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Septin 2 with ab179436 at 1/50 dilution.
Immunofluorescent analysis of MCF-7 cells labeling Septin 2 with ab179436 at 1/50 dilution (red). DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized Jurkat cells labeling Septin 2 with ab179436 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
Western blot analysis on immunoprecipitation pellet from (1) MCF-7 cell lysate or (2) 1XPBS (negative control) using ab179436 at a 1/10 dilution for IP, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.