All lanes : Anti-Septin 2 antibody - C-terminal (ab185998) at 0.1 µg/mlLane 1 : HeLa whole cell lysateLane 2 : 293T whole cell lysateLane 3 : Jurkat whole cell lysateLane 4 : TCMK1 whole cell lysateLane 5 : NIH 3T3 whole cell lysateLysates/proteins at 50 µg per lane.developed using the ECL technique
Detection of Human Septin 2 by Western Blot of Immunoprecipitates of 293Twhole cell lysates (1 mg for IP; 20% of IP loaded).Lane 1: ab185998 used for IP at 6 μg/mg lysate.Lane 2: Control IgG.For blotting, ab185998 was used at 1 μg/ml.Detection: Chemiluminescence with an exposure time of 3 seconds.