Anti-Serine racemase antibody
| Name | Anti-Serine racemase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab45434 |
| Prices | $378.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Serine racemase |
| Description | Rabbit Polyclonal |
| Gene | SRR |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab45434 stained human SHSY5Y cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab45434, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
All lanes : Anti-Serine racemase antibody (ab45434) at 1 µg/mlLane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 2 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 3 : Brain (Mouse) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
Product References
Effects of Chronic D-Serine Elevation on Animal Models of Depression and - Effects of Chronic D-Serine Elevation on Animal Models of Depression and
Otte DM, Barcena de Arellano ML, Bilkei-Gorzo A, Albayram O, Imbeault S, Jeung H, Alferink J, Zimmer A. PLoS One. 2013 Jun 21;8(6):e67131. Print 2013.
Nicotinic acetylcholine receptor antagonists alter the function and expression of - Nicotinic acetylcholine receptor antagonists alter the function and expression of
Singh NS, Paul RK, Ramamoorthy A, Torjman MC, Moaddel R, Bernier M, Wainer IW. Cell Signal. 2013 Dec;25(12):2634-45.