ICC/IF image of ab45545 stained human SHSY5Y cells. The cells were methanol fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab45545, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
All lanes : Anti-Serine racemase antibody (ab45545) at 1 µg/mlLane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)Lane 2 : Brain (Rat) Tissue Lysate Lane 3 : Brain (Mouse) Tissue Lysate Lane 4 : Hippocampus (Rat) Tissue Lysate Lane 5 : Hippocampus (Mouse) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.Observed band size : 37 kDa (why is the actual band size different from the predicted?)Additional bands at : 100 kDa,60 kDa,70 kDa. We are unsure as to the identity of these extra bands.