Anti-Serine racemase antibody [EPR16682]

• Supplier: Abcam
• Catalog: ab200833
• Prices: $99.00, $347.00
• Sizes: 10 µl, 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16682
• Applications: WB ICC/IF ICC/IF IP
• Species Reactivities: Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Serine racemase aa 50-150
• Description: Rabbit Monoclonal
• Gene: SRR
• Conjugate: Unconjugated

Product images

All lanes : Anti-Serine racemase antibody [EPR16682] (ab200833) at 1/5000 dilutionLane 1 : LNCaP (Human prostate cancer cell line) whole cell lysateLane 2 : Human fetal brain lysateLane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysateLane 4 : HEK293 (Human embryonic kidney) whole cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1000

Serine racemase was immunoprecipitated from 1mg of HEK293 (Human embryonic kidney) whole cell lysate with ab200833 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200833 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: HEK293 whole cell lysate 10 µg (Input). Lane 2: ab200833 IP in HEK293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200833 in HEK293 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 30 seconds

Serine racemase was immunoprecipitated from 1mg of LNCaP (Human prostate cancer cell line) whole cell lysate with ab200833 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200833 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: LNCaP whole cell lysate 10 µg (Input). Lane 2: ab200833 IP in LNCaP whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200833 in LNCaP whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 10 seconds