Anti-Serine/threonine-protein kinase 4 antibody
| Name | Anti-Serine/threonine-protein kinase 4 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57836 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB ICC/IF ICC/IF IHC-P FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein corresponding to Human Serine/threonine-protein kinase 4 aa 1-40 |
| Description | Mouse Monoclonal |
| Gene | STK4 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Serine/threonine-protein kinase 4antibody (ab57836) used in immunofluorescence at 10ug/ml on HeLa cells.
Serine/threonine-protein kinase 4antibody (ab57836) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human stomach carcinoma tissue.
![Overlay histogram showing HeLa cells stained with ab57836 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57836, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/28593_MST1-Primary-antibodies-ab57836-1.jpg)
Overlay histogram showing HeLa cells stained with ab57836 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57836, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Inactivation of the Hippo tumour suppressor pathway by integrin-linked kinase. - Inactivation of the Hippo tumour suppressor pathway by integrin-linked kinase.
Serrano I, McDonald PC, Lock F, Muller WJ, Dedhar S. Nat Commun. 2013;4:2976.
FRMD4A upregulation in human squamous cell carcinoma promotes tumor growth and - FRMD4A upregulation in human squamous cell carcinoma promotes tumor growth and
Goldie SJ, Mulder KW, Tan DW, Lyons SK, Sims AH, Watt FM. Cancer Res. 2012 Jul 1;72(13):3424-36.