CISH (D4D9) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 8731
• Prices: $246.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D4D9
• Applications: WB IP ICC/IF FC
• Species Reactivities: Human, Mouse, Monkey
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro176 of human CISH protein.
• Description: Rabbit Monoclonal
• Gene: CISH

Product images

Western blot analysis of extracts from NK-92 cells, untreated (-) or treated (+) with Human Interleukin-2 (hIL-2) #8907 (10 ng/ml, overnight), or BaF3 cells, untreated (-) or treated (+) with Mouse Interleukin-3 (mIL-3) #8923 (10 ng/ml, 6 hr), using CISH (D4D9) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing full-length human CISH (hCISH, +), using CISH (D4D9) Rabbit mAb.

Confocal immunofluorescent analysis of NK-92 cells, IL-2 starved for 5 hrs (left) or IL-2 starved for 5 hrs followed by addition of hIL-2 #8907 overnight (right), using CISH (D4D9) Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of NK-92 cells, IL-2 starved for 5 hrs (blue) or IL-2 starved for 5 hrs followed by additon of hIL-2 #8907 overnight (green), using CISH (D4D9) Rabbit mAb (green).