Anti-SET antibody
| Name | Anti-SET antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab1183 |
| Prices | $400.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IP WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide, corresponding to amino acids 3-18 of Human I2 alpha PP2A |
| Description | Rabbit Polyclonal |
| Gene | SET |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-SET antibody (ab1183) at 1/2000 dilution + HeLa cell lysate at 20 µgSecondaryAnti-rabbit IgG HRP-conjugate polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 10 secondsThis image is courtesy of an anonymous AbreviewSee Abreview
Western blot of 10 µg HEK 293 cell extracts with Anti- I2α (GLO150). The band corresponds to I2αPP2A (~ 39 kDa). Western blot of 10 µg HEK 293 cell extracts with Anti- I2a (GLO150). The band corresponds to I2aPP2A (~ 39 kDa).
ICC/IF image of ab1183 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1183, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab35252 (4µg/ml) staining SET in human kidney cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and cytoplasmic staining.Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Product References
A role for the nucleosome assembly proteins TAF-Ibeta and NAP1 in the activation - A role for the nucleosome assembly proteins TAF-Ibeta and NAP1 in the activation
Mansouri S, Wang S, Frappier L. PLoS One. 2013 May 14;8(5):e63802.
SET-NUP214 fusion in acute myeloid leukemia- and T-cell acute lymphoblastic - SET-NUP214 fusion in acute myeloid leukemia- and T-cell acute lymphoblastic
Quentmeier H, Schneider B, Rohrs S, Romani J, Zaborski M, Macleod RA, Drexler HG. J Hematol Oncol. 2009 Jan 23;2:3.
Proteomic analysis of SET-binding proteins. - Proteomic analysis of SET-binding proteins.
Vera J, Estanyol JM, Canela N, Llorens F, Agell N, Itarte E, Bachs O, Jaumot M. Proteomics. 2007 Feb;7(4):578-87.
Glyceraldehyde 3-phosphate dehydrogenase is a SET-binding protein and regulates - Glyceraldehyde 3-phosphate dehydrogenase is a SET-binding protein and regulates
Carujo S, Estanyol JM, Ejarque A, Agell N, Bachs O, Pujol MJ. Oncogene. 2006 Jul 6;25(29):4033-42. Epub 2006 Feb 13.
Heterogeneous nuclear ribonucleoprotein A2 is a SET-binding protein and a PP2A - Heterogeneous nuclear ribonucleoprotein A2 is a SET-binding protein and a PP2A
Vera J, Jaumot M, Estanyol JM, Brun S, Agell N, Bachs O. Oncogene. 2006 Jan 12;25(2):260-70.