SET

Gene Symbol SET
Entrez Gene 6418
Alt Symbol 2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, TAF-I, TAF-IBETA
Species Human
Gene Type protein-coding
Description SET nuclear proto-oncogene
Other Description HLA-DR-associated protein II|SET nuclear oncogene|SET translocation (myeloid leukemia-associated)|Template-Activating Factor-I, chromatin remodelling factor|inhibitor of granzyme A-activated DNase|inhibitor-2 of protein phosphatase-2A|phosphatase 2A inhibitor I2PP2A|protein SET|protein phosphatase type 2A inhibitor
Swissprots B4DUE2 Q5VXV1 A6NGV1 Q5VXV2 Q15541 Q01105 Q6FHZ5 A5A5H4
Accessions EAW87818 EAW87819 EAW87820 EAW87821 EAW87822 EAW87823 Q01105 AK223556 BAD97276 AK300609 BAG62304 AK300794 BAG62453 AK311346 AK315313 BAG37717 AM392720 CAL37598 AM392742 CAL37620 AM393022 CAL37900 AM393175 CAL38053 AM393446 CAL38324 AY349172 AAQ79833 BC014567 BC032749 AAH32749 BP343325 BQ774097 BU543146 CA392072 CA419540 CB129868 CN408133 CR536543 CAG38780 CR542050 CAG46847 D45198 BAA08139 DQ894408 ABM85334 EF534308 ABP96841 EU176263 ABW03714 JF729356 AEO27880 M93651 AAA60318 U51924 AAC50460 X75091 CAA52982 XM_011518911 XP_011517213 NM_001122821 NP_001116293 NM_001248000 NP_001234929 NM_001248001 NP_001234930 NM_003011 NP_003002
Function Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T- lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher. {ECO:0000269|PubMed:11555662, ECO:0000269|PubMed:12628186}.
Subcellular Location Cytoplasm, cytosol. Endoplasmic reticulum. Nucleus, nucleoplasm. Note=In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. The SET complex is associated with the endoplasmic reticulum. Following CTL attack and cleavage by GZMA, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium.
Tissue Specificity Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.

I2PP2A (E-15) - sc-5655 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
I2PP2A (E-15): sc-5655. Western blot analysis of I2PP2A expression in Jurkat (A) and K-562 (B) nuclear extracts.

I2PP2A (H-120) - sc-25564 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
I2PP2A (H-120): sc-25564. Western blot analysis of human recombinant I2PP2A fusion protein.

I2PP2A (F-17) - sc-27269 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
I2PP2A (F-17): sc-27269. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing nuclear staining of cells in glomeruli and nuclear and cytoplasmic staining of cells in tubules.

I2PP2A (F-9) - sc-133138 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
I2PP2A (F-9): sc-133138. Western blot analysis of I2PP2A expression in SK-N-MC (A) and KNRK (B) whole cell lysates.

Anti-SET antibody - ab1183 from Abcam

Prices $400.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-SET antibody (ab1183) at 1/2000 dilution + HeLa cell lysate at 20 µgSecondaryAnti-rabbit IgG HRP-conjugate polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 10 secondsThis image is courtesy of an anonymous AbreviewSee Abreview

Anti-SET antibody - ab85389 from Abcam

Prices $394.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SET antibody (ab85389) at 1 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-SET antibody - ab92872 from Abcam

Prices $394.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SET with ab92872 at 1/400 (0.5µg/ml). A DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.

Anti-SET antibody - ab97596 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-SET antibody (ab97596) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

Anti-SET antibody - ab1339 from Abcam

Prices $378.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
SET staining (2µg/ml) of Human Kidney extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

Anti-SET antibody [EPR12972(B)] - ab176567 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12972(B)
All lanes : Anti-SET antibody [EPR12972(B)] (ab176567) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : 293T cell lysateLane 3 : HepG2 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Anti-SET antibody [EPR12973] - ab181990 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12973
All lanes : Anti-SET antibody [EPR12973] (ab181990) at 1/100000 dilutionLane 1 : Jurkat cell lysateLane 2 : 293T cell lysateLane 3 : Hela cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Anti-SET / TAF-I Antibody - LS-C343384 from LifeSpan Bioscience

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TAF1A Antibody (Arg318) - LS-C176935 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of TAF I p48 (R318) pAb in paraffin-embedded human brain carcinoma tissue.

Anti-SET / TAF-I Antibody (clone M1-F5) - LS-C198177 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone M1-F5
Immunofluorescence of monoclonal antibody to SET on HeLa cell . [antibody concentration 10 ug/ml]

Anti-SET / TAF-I Antibody (aa46-75) - LS-C169000 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
SET Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-SET / TAF-I Antibody (aa175-225) - LS-C289017 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human SET by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-SET used at a dilution of 1:200 (1 ug/ml). Detection: DAB.

Anti-SET / TAF-I Antibody - LS-C334566 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using SET antibody.

Anti-SET / TAF-I Antibody (aa66-81) IHC-plus™ - LS-B2058 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-SET antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2058 concentration 10 ug/ml.

Anti-SET / TAF-I Antibody (aa125-175) - LS-C289014 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human SET by Immunofluorescence. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SET used at a dilution of 1:400 (0.5 ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated (A120-601D4) used at a dilution of 1:100.

Anti-SET / TAF-I Antibody (aa125-175) - LS-C286854 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human SET by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-SET used at a dilution of 1:250.

Anti-SET / TAF-I Antibody IHC-plus™ - LS-B9483 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-SET / TAF-I antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-SET / TAF-I Antibody (aa1-45) - LS-C185797 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded A549 xenograft using SET antibody at 1:100 dilution.

Anti-SET / TAF-I Antibody (aa2-15) IHC-plus™ - LS-B4032 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-SET antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4032 concentration 3.75 ug/ml.

Anti-SET / TAF-I Antibody - LS-C18615 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-SET / TAF-I Antibody (aa3-16) - LS-C39076 from LifeSpan Bioscience

Prices $385.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
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