All lanes : Anti-SET antibody [EPR12972(B)] (ab176567) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : 293T cell lysateLane 3 : HepG2 cell lysateLane 4 : HeLa cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique
Flow cytometry analysis of permeabilized Jurkat cells labeling SET (red) with ab176567 at a 1/10 dilution, or negative control rabbit IgG (green)
Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling SET with ab176567 at 1/10 dilution.