Anti-SETD7 antibody [s4E5] - ChIP Grade
| Name | Anti-SETD7 antibody [s4E5] - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab14820 |
| Prices | $387.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Clone | s4E5 |
| Applications | ELISA WB ChIP IHC-P IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Recombinant full length protein (Human) |
| Description | Mouse Monoclonal |
| Gene | SETD7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunoflouresence was carried out using mouse cells in culture. Conditions: 4% PFA fixation and dilution with 0.5% triton, 10mg/ml BSA in PBS. Dilution of ab14820 used: 1/50. Green = ab14820 detected with secondary antibody (Alexa 488). Blue = DAPI stain.
ab14820 at 1/25 dilution staining KMT7 / SETD7 / SET7/9 in mouse pancrease tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 5% serum for 1 hour at 20°C and then incubated with ab14820 for 18 hours at 4°C. A Cy2 conjugated donkey polyclonal to mouse Ig, diluted 1/20, was used as the secondary antibody.See Abreview
![Overlay histogram showing HeLa cells stained with ab14820 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14820, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/28910_SETD7-Primary-antibodies-ab14820-5.jpg)
Overlay histogram showing HeLa cells stained with ab14820 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14820, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
The histone methyltransferase Set7/9 promotes myoblast differentiation and - The histone methyltransferase Set7/9 promotes myoblast differentiation and
Tao Y, Neppl RL, Huang ZP, Chen J, Tang RH, Cao R, Zhang Y, Jin SW, Wang DZ. J Cell Biol. 2011 Aug 22;194(4):551-65.
Peroxisome proliferator-activated receptor gamma activation restores islet - Peroxisome proliferator-activated receptor gamma activation restores islet
Evans-Molina C, Robbins RD, Kono T, Tersey SA, Vestermark GL, Nunemaker CS, Garmey JC, Deering TG, Keller SR, Maier B, Mirmira RG. Mol Cell Biol. 2009 Apr;29(8):2053-67.