Anti-SF20 antibody [EPR13211] (ab181096) at 1/1000 dilution + Human placenta lysate at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution (Goat Anti-Rabbit IgG HRP)
Anti-SF20 antibody [EPR13211] (ab181096) at 1/2000 dilution + MCF7 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow cytometric analysis of MCF7 cells (2% paraformaldehyde-fixed) labeling SF20 with ab181096 at 1/30 dilution (red) . A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Isotype control: Rabbit monoclonal IgG (green).