Anti-SFRP2 antibody
| Name | Anti-SFRP2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab92667 |
| Prices | $384.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | Synthetic peptide corresponding to an internal sequence of Human SFRP2 |
| Description | Rabbit Polyclonal |
| Gene | SFRP2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab92667 stained SKNSH cells. The cells were 4% fomaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92667, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-SFRP2 antibody (ab92667) at 1/500 dilutionLane 1 : HuvEc cell lysate Lane 2 : COLO cell lysate Lane 3 : HeLa cell lysate Lane 4 : HeLa cell lysate with immunizing peptide at 10 µgLysates/proteins at 30 µg per lane.
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