Anti-SGK1 (phospho S422) antibody
| Name | Anti-SGK1 (phospho S422) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55281 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic phosphopeptide derived from human SGK1 around the phosphorylation site of serine 422 (G-F-SP-Y-A) |
| Description | Rabbit Polyclonal |
| Gene | SGK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-SGK1 (phospho S422) antibody (ab55281) at 1/500 dilutionLane 1 : extracts from HeLa cells treated with Insulin (0.01U/ml, 15mins).Lane 2 : extracts from HeLa cells treated with Insulin (0.01U/ml, 15mins), and incubated with the immunising phosphopeptide.
Immunohistochemical analysis of SGK1 (phospho S422) expression in human breast carcinoma tissue using 1/50 ab55281. Left: no phosphopeptide. Right: sample treated with immunising phosphopeptide.
ICC/IF image of ab55281 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55281, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Evidence that TSC2 acts as a transcription factor and binds to and represses the - Evidence that TSC2 acts as a transcription factor and binds to and represses the
Pradhan SA, Rather MI, Tiwari A, Bhat VK, Kumar A. Nucleic Acids Res. 2014 Jun;42(10):6243-55.
Serum- and glucocorticoid-regulated kinase 1 is required for nuclear export of - Serum- and glucocorticoid-regulated kinase 1 is required for nuclear export of
Alamares-Sapuay JG, Martinez-Gil L, Stertz S, Miller MS, Shaw ML, Palese P. J Virol. 2013 May;87(10):6020-6.
Ethanol regulation of serum glucocorticoid kinase 1 expression in DBA2/J mouse - Ethanol regulation of serum glucocorticoid kinase 1 expression in DBA2/J mouse
Costin BN, Dever SM, Miles MF. PLoS One. 2013 Aug 22;8(8):e72979.
Increased expression of serum- and glucocorticoid-regulated kinase-1 in the - Increased expression of serum- and glucocorticoid-regulated kinase-1 in the
Szebeni B, Vannay A, Sziksz E, Prokai A, Cseh A, Veres G, Dezsofi A, Gyorffy H, Szabo IR, Arato A. J Pediatr Gastroenterol Nutr. 2010 Feb;50(2):147-53. doi: