Anti-SGK1 antibody
| Name | Anti-SGK1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab59337 |
| Prices | $359.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA IHC-F IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthesized non-phosphopeptide derived from human SGK1 around the phosphorylation site of serine 78 (P-P-SP-P-S) |
| Description | Rabbit Polyclonal |
| Gene | SGK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-SGK1 antibody (ab59337) at 1/500 dilutionLane 1 : 293 cells treated with heat shockLane 2 : 293 cells treated with heat shock with immunizing non-phosphopeptide
ab59337, at a 1/50-1/100 dilution, staining SGK1 in Human brain sections.
ab59337, at a 1/50-1/100 dilution, staining SGK1 in Human brain sections, in the presence of the immunizing peptide.
ICC/IF image of ab59337 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59337, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Role for the kinase SGK1 in stress, depression, and glucocorticoid effects on - Role for the kinase SGK1 in stress, depression, and glucocorticoid effects on
Anacker C, Cattaneo A, Musaelyan K, Zunszain PA, Horowitz M, Molteni R, Luoni A, Calabrese F, Tansey K, Gennarelli M, Thuret S, Price J, Uher R, Riva MA, Pariante CM. Proc Natl Acad Sci U S A. 2013 May 21;110(21):8708-13. doi:
Ethanol regulation of serum glucocorticoid kinase 1 expression in DBA2/J mouse - Ethanol regulation of serum glucocorticoid kinase 1 expression in DBA2/J mouse
Costin BN, Dever SM, Miles MF. PLoS One. 2013 Aug 22;8(8):e72979.
Mechanisms for acute stress-induced enhancement of glutamatergic transmission and - Mechanisms for acute stress-induced enhancement of glutamatergic transmission and
Yuen EY, Liu W, Karatsoreos IN, Ren Y, Feng J, McEwen BS, Yan Z. Mol Psychiatry. 2011 Feb;16(2):156-70.
The dissection of transcriptional modules regulated by various drugs of abuse in - The dissection of transcriptional modules regulated by various drugs of abuse in
Piechota M, Korostynski M, Solecki W, Gieryk A, Slezak M, Bilecki W, Ziolkowska B, Kostrzewa E, Cymerman I, Swiech L, Jaworski J, Przewlocki R. Genome Biol. 2010;11(5):R48.
Rescue of DeltaF508-CFTR by the SGK1/Nedd4-2 signaling pathway. - Rescue of DeltaF508-CFTR by the SGK1/Nedd4-2 signaling pathway.
Caohuy H, Jozwik C, Pollard HB. J Biol Chem. 2009 Sep 11;284(37):25241-53.