Anti-SGK1 antibody [Y238]
| Name | Anti-SGK1 antibody [Y238] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32374 |
| Prices | $387.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | Y238 |
| Applications | WB IHC-P FC IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues of human SGK1 |
| Description | Rabbit Monoclonal |
| Gene | SGK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-SGK1 antibody [Y238] (ab32374) at 1/500 dilutionLane 1 : A431 cell lysateLane 2 : NIH3T3 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/29294_ab32374_1.JPG)
All lanes : Anti-SGK1 antibody [Y238] (ab32374) at 1/500 dilutionLane 1 : A431 cell lysateLane 2 : NIH3T3 cell lysate
Ab32374, at a 1/50 dilution, staining SGK1 in paraffin embedded human kidney carcinoma tissue sections by Immunohistochemistry.
Overlay histogram showing HEK293 cells stained with ab32374 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32374, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Low SGK1 expression in human adrenocortical tumors is associated with - Low SGK1 expression in human adrenocortical tumors is associated with
Ronchi CL, Sbiera S, Leich E, Tissier F, Steinhauer S, Deutschbein T, Fassnacht M, Allolio B. J Clin Endocrinol Metab. 2012 Dec;97(12):E2251-60.
Subcellular location of serum- and glucocorticoid-induced kinase-1 in renal and - Subcellular location of serum- and glucocorticoid-induced kinase-1 in renal and
Cordas E, Naray-Fejes-Toth A, Fejes-Toth G. Am J Physiol Cell Physiol. 2007 May;292(5):C1971-81. Epub 2007 Jan 3.