All lanes : Anti-SH3PX1 antibody [2F1] (ab118996) at 1/1000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY SH3PX1 cDNALysates/proteins at 5 µg per lane.
All lanes : Anti-SH3PX1 antibody [2F1] (ab118996) at 1/1000 dilutionLane 1 : HepG2 cell line extractLane 2 : HeLa cell line extractLane 3 : HT29 cell line extractLane 4 : A549 cell line extractLane 5 : COS7 cell line extractLane 6 : Jurkat cell line extractLane 7 : MDCK cell line extractLane 8 : PC12 cell line extractLane 9 : MCF7 cell line extractLysates/proteins at 35 µg per lane.
ab118996, at 1/100 dilution staining SH3PX1 in COS7 cells transiently transfected with pCMV6-ENTRY SH3PX1 by Immunofluorescence.
ab118996, at 1/100, staining SH3PX1 in HEK293T cells transfected with either SH3PX1 overexpress plasmid (Red) or empty vector control plasmid (Blue) by Flow Cytometry.
ab118996, at 1/100, staining SH3PX1, in Jurkat cells by Flow cytometry (Red), compared to a nonspecific negative control antibody (Blue).