Western blot analysis of HepG2 cell lysate immunoprecipitated using ab181856 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody was used at 1/1000 dilution.
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling SH3PX1 with ab181856 at 1/10 dilution (red) compared to a Rabbit IgG monoclonal isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Immunofluorescent analysis of 4% paraformaldehyde-fixed HCT-116 cells labeling SH3PX1 with ab181856 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Immunofluorescent analysis of acetone-fixed HeLa cells labeling SH3PX1 with ab181856 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SH3PX1 with ab181856 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Anti-SH3PX1 antibody [EPR14399] (ab181856) at 1/10000 dilution + HCT-116 cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Anti-SH3PX1 antibody [EPR14399] (ab181856) at 1/50000 dilution + HeLa cell lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution