Anti-SHARP1 antibody - N-terminal
| Name | Anti-SHARP1 antibody - N-terminal |
|---|---|
| Supplier | Abcam |
| Catalog | ab175544 |
| Prices | $370.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | FC WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide within Human SHARP1 aa 24-45 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | BHLHE41 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-SHARP1 antibody - N-terminal (ab175544) at 1/1000 dilution + RD cell lysate at 35 µg
Fluorescent confocal image of MCF-7 cell stained with ab175544 at 1/25 dilution. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab175544 (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SHARP1 immunoreactivity is localized to nucleus significantly.
Flow cytometric analysis of NCI-H460 cells (right histogram) labeling SHARP1 at 1/10 dilution compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.