Western blot analysis with ab197853 of Immunoprecipitationusing U2OS cells extractsLane 1: Input.Lane 2: Immunoprecipitation with control serum.Lane 3: Immunoprecipitation with ab197853 (5μl)
All lanes : Anti-SHARPIN antibody (ab197853) at 1/2000 dilutionLane 1 : Control siRNA transfected U2OS cell lysateLane 2 : Sharpin siRNA transfected U2OS cell lysateLane 3 : NIH3T3 cell lysateLysates/proteins at 40 µg per lane.
Flow cytometric analysis of U2OS cells labeling Sharpin with ab197853 at 1/200 dilution. Upper panel shows labeling wit IgG controls, middle panel shows labeling of control siRNA treated cells and bottom panel shows labeling of Sharpin siRNA treated cells. Cells were fixed with 1% PFA. Cells were incubated with the primary antibody overnight at 4°C.
Immunofluorescent analysis of PFA fixed and methanol permeabilizaed U2OS cells transfected with HA-Sharpin labeling Sharpin with ab197853 at 1/200 dilution in PBST (green) and HA in (red)